Key result
The minor allele of rs17427875 (AT genotype) in lncRNA-HOXA11-AS was associated with a lower survival rate in patients with subarachnoid hemorrhage compared to the AA genotype.
Why the study?
HOAX11-AS promotes liver cancer progression via miR-15a-3p/STAT3, but the effect of rs17427875 on the prognosis and molecular mechanisms of subarachnoid hemorrhage was unknown.
Does the rs17427875 polymorphism in lncRNA-HOXA11-AS influence survival and inflammation in patients with subarachnoid hemorrhage?
Cohort (n=158)
Does the rs17427875 polymorphism in lncRNA-HOXA11-AS influence survival and inflammation in patients with subarachnoid hemorrhage?
The rs17427875 polymorphism in lncRNA-HOXA11-AS may serve as a prognostic biomarker in subarachnoid hemorrhage, with the AT genotype associated with worse survival and increased inflammation.
Answers are grounded in this paper and cite their sources.
May inform subarachnoid hemorrhage prognosis; hypothesis-generating for rs17427875 as biomarker, pending prospective validation.
Background: HOAX11-AS was reported to promote the progression of liver cancer via the signaling pathway of miR-15a-3p/STAT3. In this study, we investigated the effect of rs17427875 on the prognosis of subarachnoid hemorrhage (SAH) and its underlying molecular mechanisms. Methods: 158 SAH patients were recruited and grouped according to their genotypes rs17427875. Peripheral blood and cerebrospinal fluid (CSF) samples were collected for subsequent analysis. Quantitative real-time PCR, luciferase assays, Western blot and ELISA were performed to analyze the correlations between the expression of lncRNA-HOXA11-AS, miR-15a, TNF-α and NF-κB. Results: The survival rate was remarkably higher in SAH patients carrying the AA genotype of rs17427875 when compared with those carrying the AT genotype. The expression of miR-15a was significantly repressed in the peripheral blood and CSF of SAH patients carrying the AT allele when compared with that in patients carrying the AA allele. MiR-15a showed a remarkable efficacy in inhibiting the luciferase activity of wild type lncRNA-HOXA11-AS and STAT3 in THP-1 cells. P-HOXA11-AS-T showed a stronger ability to suppress the expression of miR-15a and activate the expression of STAT3, TNF-α and NF-κB in THP-1 cells when compared with P-HOXA11-AS-A. Conclusions: The findings demonstrated that the presence of the minor allele of rs17427875 in lncRNA-HOXA11-AS could increase the expression level of lncRNA-HOXA11-AS, thus elevating the expression level of STAT3 via down-regulating miR-15a, and increased STAT3 expression could aggravate inflammation to cause poor prognosis of SAH. Therefore, the rs17427875 polymorphism can be used as a potential biomarker for the prognosis of SAH.
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Zhou et al. (2022) conducted a cohort in subarachnoid hemorrhage (SAH) (n=158). rs17427875 AT genotype (minor allele) vs. rs17427875 AA genotype was evaluated on Survival rate. The minor allele of rs17427875 (AT genotype) in lncRNA-HOXA11-AS was associated with a lower survival rate in patients with subarachnoid hemorrhage compared to the AA genotype.
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