What question did this study set out to answer?

To investigate the molecular mechanisms by which T-cell lymphokines IL-2 and IL-5 activate transcription of the J-chain gene in B cells.

December 15, 1991Proceedings of the National Academy of SciencesOpen Access

Interleukin 2- and interleukin 5-induced changes in the binding of regulatory factors to the J-chain gene promoter.

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Authors

HMH J McFaddenOregon State University MKM E KoshlandNew York University

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Implication

In vitro study demonstrates IL-2 and IL-5 reduce repressor binding at the J-chain promoter in lymphoma B cells, suggesting convergent signaling drives IgM joining component expression.

Key Points

To investigate the molecular mechanisms by which T-cell lymphokines IL-2 and IL-5 activate transcription of the J-chain gene in B cells.
Assayed J-chain RNA abundance and nuclear factor binding at the J-chain promoter using an inducible B-lymphoma cell line model.
Tested cellular responses under stimulation with IL-2 and IL-5 individually, in combination, following lymphokine withdrawal, and in the presence of IL-4.
Both IL-2 and IL-5 prompted a decrease in the binding of two promoter-specific nuclear proteins, an event occurring prior to J-chain RNA transcription.
Combined treatment with IL-2 and IL-5 produced nearly additive changes in factor binding reduction and J-chain RNA expression.
Observed regulatory changes were reversed after stimulus withdrawal and were suppressed in the presence of IL-4.

Cite This Study

McFadden et al. (1991) studied this question.

synapsesocial.com/papers/6a9837b9161d47660415fc97 https://doi.org/10.1073/pnas.88.24.11027

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Implication

In vitro study demonstrates IL-2 and IL-5 reduce repressor binding at the J-chain promoter in lymphoma B cells, suggesting convergent signaling drives IgM joining component expression.

Interleukin 2- and interleukin 5-induced changes in the binding of regulatory factors to the J-chain gene promoter.

Discussion

Member takes

Implication

Key Points

Cite This Study

Interleukin 2- and interleukin 5-induced changes in the binding of regulatory factors to the J-chain gene promoter.

Discussion

Member takes

Implication

Key Points

Cite This Study

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