Chemicals-Components of the media were purchased from Sigma Chemical Co., St. Louis, MO, except the yeast extract which was from Difco Laboratories, Detroit, MI, and trypticase which was from BBL, CockeysviUe, MD.MiUipore filters and adsorbent pads were purchased from Millipore
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Dimond et al. (1981) studied this question.
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