What question did this study set out to answer?

To determine the subcellular localization and interaction of glucokinase and glucokinase regulatory protein in hepatocytes under simulated feeding and fasting conditions.

February 1, 1997Diabetes

Glucokinase Regulatory Protein May Interact With Glucokinase in the Hepatocyte Nucleus

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Authors

KBKaren BrownUniversity of Leicester SKStephen KalinowskiBristol-Myers Squibb (Germany)JMJohn R. MegillBristol-Myers Squibb (United States)

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Implication

Experimental study demonstrates nutrient-dependent glucokinase translocation from the nucleus in hepatocytes, suggesting a broader role in hepatic energy metabolism.

Key Points

To determine the subcellular localization and interaction of glucokinase and glucokinase regulatory protein in hepatocytes under simulated feeding and fasting conditions.
Examined the intracellular distribution of glucokinase regulatory protein and glucokinase in liver cells exposed to low versus high glucose concentrations.
Assessed changes in protein localization following stimulation with low concentrations of fructose and subsequent exposure to glucagon.
Glucokinase regulatory protein remained localized primarily in the hepatocyte nucleus under conditions simulating both fed and fasted states.
Glucokinase localized to the nucleus at low glucose concentrations and translocated to the cytoplasm at high glucose or low fructose concentrations, with fructose producing a more rapid and robust effect.
Glucagon partially reversed the translocation of glucokinase from the nucleus to the cytoplasm induced by high glucose and fructose.

Cite This Study

Brown et al. (1997) studied this question.

synapsesocial.com/papers/6a98495fb66eb566e1faa142 https://doi.org/10.2337/diab.46.2.179

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Implication

Experimental study demonstrates nutrient-dependent glucokinase translocation from the nucleus in hepatocytes, suggesting a broader role in hepatic energy metabolism.

Glucokinase Regulatory Protein May Interact With Glucokinase in the Hepatocyte Nucleus

Discussion

Member takes

Implication

Key Points

Cite This Study

Glucokinase Regulatory Protein May Interact With Glucokinase in the Hepatocyte Nucleus

Discussion

Member takes

Implication

Key Points

Cite This Study

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