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Methylotrophic bacteria, isolated from soil samples or from sewage sludge, proved to be useful sources of esterases for catalyzing the enantioselective hydrolysis of racemic N-acetyl-indoline-2-carboxylic acid methyl ester (7) to the corresponding (25) or (2R)-N- acetyl amino acid (6) with high optical yields. From the DMF-utilizer Pseudomonas DMF 5/8 and the methanol-utilizer Isolate EE 210, the corresponding esterases were isolated. Reactions with whole cells as well as with the purified enzymes are described.
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Tombo et al. (1987) studied this question.
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