What question did this study set out to answer?

To determine the dynamics, ultrastructure, and functional contribution of central-spindle microtubules to chromosome segregation during anaphase.

July 24, 2019Molecular Biology of the Cell

Central-spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B

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To determine the dynamics, ultrastructure, and functional contribution of central-spindle microtubules to chromosome segregation during anaphase.
Tracked central-spindle microtubule dynamics during chromosome segregation in human mitotic spindles as well as Caenorhabditis elegans mitotic and female meiotic spindles.
Applied targeted laser ablation to disrupt central-spindle microtubules and evaluate the immediate mechanical effect on chromosome movement.
Used electron tomographic reconstruction to map microtubule architecture and endpoint locations within the anaphase central spindle.
Central-spindle microtubules slid apart at the exact same velocity as segregating chromosomes across all spindle models evaluated, including during poleward chromosome movement.
Laser ablation of central-spindle microtubules caused an immediate and complete arrest of chromosome motion.
Electron tomographic reconstructions demonstrated that anaphase spindles contain distinct microtubules with both ends terminating entirely between segregating chromosomes.

Authors

CYChe‐Hang YuUniversity of California, Santa Barbara SRStefanie RedemannUniversity of Virginia HWHai‐Yin WuHarvard University Press

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Implication

Cellular imaging study reveals strong coupling between central-spindle microtubules and chromosomes in human and nematode spindles, indicating central spindles actively drive segregation.

Cite This Study

Yu et al. (2019) studied this question.

synapsesocial.com/papers/6a9869c478eff8dc11f37cb6 https://doi.org/10.1091/mbc.e19-01-0074

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Implication

Cellular imaging study reveals strong coupling between central-spindle microtubules and chromosomes in human and nematode spindles, indicating central spindles actively drive segregation.

Central-spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B

Key Points

Discussion

Implication

Cite This Study

Central-spindle microtubules are strongly coupled to chromosomes during both anaphase A and anaphase B

Key Points

Discussion

Implication

Cite This Study

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