Key result
In a mouse model of hemolytic uremic syndrome, glomerular VEGF and Ang-1 expression increased during the recovery phase, suggesting they play important roles in regenerating endothelial injury.
VEGF and Ang-1 appear to play important roles in the regeneration of endothelial injury during recovery from hemolytic uremic syndrome in a mouse model.
May guide mechanistic studies of endothelial repair in HUS; leaves open translation to human disease and therapy.
BACKGROUND/AIMS: The recovery process from renal injury in hemolytic uremic syndrome (HUS) remains obscure. In order to clarify the role of vascular endothelial growth factor (VEGF) and angiopoietin 1 (Ang-1) in the renal recovery from HUS, we produced a model of mild HUS and examined the renal recovery process. METHODS: We investigated three groups of mice. Group 1 consisted of mice that received an injection of Shiga toxin 2 (Stx2) and lipopolysaccharide (LPS); group 2 consisted of mice that received an injection of low dose of Stx2 and LPS, and group 3 consisted of control mice. RESULTS: Serum Cr levels in group 1 were greater than those in group 2, and all mice in group 1 died, whereas all mice in group 2 remained alive. Endothelial injury at 24 h in group 1 was higher than in group 2. Electron-microscopic findings demonstrated that the endothelial cells formed immature capillary-like lumina from 7 to 28 days with increases in the expression of CD31-positive cells. Glomerular VEGF expression decreased at 72 h in group 1, but gradually increased in group 2. Glomerular Ang-1 expression peaked from 72 h to 28 days. Ang-1 expression was frequently found in the endothelial cell region of vesicle walls simultaneous with increased CD31-positive staining. CONCLUSION: Our findings suggest that VEGF and Ang-1 play important roles in the recovery process, particularly in the regeneration of endothelial injury.
No takes yet. Share an insight, caveat, or question.
Ohara et al. (2012) studied Hemolytic uremic syndrome. Shiga toxin 2 (Stx2) and lipopolysaccharide (LPS) vs. Control mice was evaluated on Renal recovery process (endothelial injury, VEGF and Ang-1 expression). In a mouse model of hemolytic uremic syndrome, glomerular VEGF and Ang-1 expression increased during the recovery phase, suggesting they play important roles in regenerating endothelial injury.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: