Key result
LncRNA HIF1A-AS2 expression and hypoxia/angiogenesis biomarkers were significantly increased in diabetic patients, peaking in those with proliferative diabetic retinopathy.
Why the study?
Dysregulation of lncRNAs drives diverse human diseases including diabetes, prompting evaluation of the role of lncRNA HIF1A-AS2 and its interplay with hypoxia, oxidative stress, and angiogenesis in diabetic retinopathy.
Observational (n=75)
LncRNA HIF1A-AS2 expression is elevated in diabetic retinopathy and correlates with hypoxia, oxidative stress, and angiogenesis markers.
LncRNA HIF1A-AS2 may warrant biomarker exploration in diabetic retinopathy; case-control data leaves open its clinical or therapeutic utility.
BACKGROUND: Diabetic retinopathy (DR) signifies a frequent serious diabetic complication influencing retinal structure and function. Dysregulation of lncRNAs drives a wide array of human diseases especially diabetes; thus, we aimed to study lncRNA HIF1A-AS2 role and its interplay with hypoxia, oxidative stress (OS), and angiogenesis in DR. MATERIALS AND METHODS: 60 DM patients in addition to 15 healthy subjects. were enrolled. LncRNA HIF1A-AS2 mRNA relative gene expression was assessed. Hypoxia inducible factor 1-alpha (HIF-1α), vascular endothelial growth factor (VEGF), mitogen activated protein kinase (MAPK), and endoglin levels were assessed. Detection of DNA damage using comet assay, and Redox status parameters were also detected. RESULTS: LncRNA HIF1A-AS2 expression was significantly increased in diabetic patients with the highest levels in proliferative DR patients. Moreover, HIFα, VEGF, MAPK, and Endogolin levels were significantly higher in the diabetic patients compared to control group with the highest levels in in proliferative DR patients. Significant DNA damage in comet assay was observed to be the highest in this group. CONCLUSION: We observed for the first time the imminent role of long noncoding RNA HIF1A-AS2 in DR throughout its stages and its interplay with hypoxia, OS, and angiogenesis via MAPK/VEGF-dependent pathway.
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Atef et al. (2022) conducted an observational in Diabetic retinopathy (n=75). Diabetes mellitus and diabetic retinopathy vs. Healthy subjects was evaluated on LncRNA HIF1A-AS2 expression and levels of HIF-1α, VEGF, MAPK, and endoglin. LncRNA HIF1A-AS2 expression and hypoxia/angiogenesis biomarkers were significantly increased in diabetic patients, peaking in those with proliferative diabetic retinopathy.
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