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November 11, 2025Experimental Eye ResearchOpen Access

Suppression of HIF-1α alleviates the symptoms associated with diabetic retinopathy in mice

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Authors

JWJing WangSFShufen FangJWJue Wang

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Overview

Treatment with PX-478 improves blood-retinal barrier integrity in diabetic retinopathy mice, indicating potential therapies.

Key Points

  • This research investigates the role of HIF-1α in diabetic retinopathy and its connection to retinal structural changes.
  • Mice were induced with diabetes using streptozotocin (STZ).
  • Retinal evaluations were conducted using H&E staining and Fundus Fluorescein Angiography (FFA).
  • HIF-1α levels and other proteins were analyzed through Western blot.
  • Evans Blue staining assessed blood-retinal barrier function post-treatment with PX-478.
  • Transmission electron microscopy (TEM) investigated autophagy in retina cells.
  • Significant up-regulation of HIF-1α and cellular senescence markers (p16, p21) was observed in diabetic mice.
  • Treatment with PX-478 blocked decreasing trends of neuroprotective factors like GDNF and SIRT1.
  • PX-478 improved blood-retinal barrier integrity, indicated by Evans Blue staining.
  • TEM showed increased autolysosomes in retinal tissues following HIF-1α inhibition.

Cite This Study

Wang et al. (2025) studied this question.

synapsesocial.com/papers/69252e9ec0ce034ddc35650ehttps://doi.org/10.1016/j.exer.2025.110741
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