What question did this study set out to answer?

To evaluate the binding, internalization, and surface redistribution dynamics of anti-glycoprotein IIb/IIIa autoantibodies in unfixed platelets from patients with idiopathic thrombocytopenic purpura.

January 1, 1992Autoimmunity

Anti-Glycoprotein Iib/Iiia Autoantibodies are Reversibly Internalized Into Platelets in Idiopathic (Autoimmune) Thrombocytopenic Purpura

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Authors

SNShosaku� NomuraKurume University MYMutsumasa YanabuKansai Medical University TFTsutomu FukuroiKansai Medical University

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Implication

In vitro study demonstrates reversible internalization and surface recycling of anti-GPIIb/IIIa antibodies in platelets, indicating an intracellular autoantibody reservoir in thrombocytopenia.

Key Points

To evaluate the binding, internalization, and surface redistribution dynamics of anti-glycoprotein IIb/IIIa autoantibodies in unfixed platelets from patients with idiopathic thrombocytopenic purpura.
Screened plasma from 64 patients with idiopathic thrombocytopenic purpura for anti-glycoprotein IIb/IIIa autoantibodies using antigen-capture ELISA and immunoblotting.
Measured platelet-binding IgG levels on unfixed platelets incubated with patient plasma and stimulated with 1 µM ADP or treated with EDTA using flow cytometry.
Anti-glycoprotein IIb/IIIa antibodies were detected in 13 of 64 patients (20.3%), with antibody-positive plasma producing significantly greater increases in platelet-binding IgG upon 1 µM ADP stimulation compared to antibody-negative plasma.
Platelet activation by 1 µM ADP caused weak alpha-granule release measured by GMP-140, whereas removal of extracellular calcium or dissociation of GPIIb/IIIa complexes with EDTA markedly inhibited the ADP-driven increase in surface platelet-binding IgG.

Cite This Study

Nomura et al. (1992) studied this question.

synapsesocial.com/papers/6a9882d43c21b72b86ffae56 https://doi.org/10.3109/08916939209001914

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Implication

In vitro study demonstrates reversible internalization and surface recycling of anti-GPIIb/IIIa antibodies in platelets, indicating an intracellular autoantibody reservoir in thrombocytopenia.

Anti-Glycoprotein Iib/Iiia Autoantibodies are Reversibly Internalized Into Platelets in Idiopathic (Autoimmune) Thrombocytopenic Purpura

Discussion

Member takes

Implication

Key Points

Cite This Study

Anti-Glycoprotein Iib/Iiia Autoantibodies are Reversibly Internalized Into Platelets in Idiopathic (Autoimmune) Thrombocytopenic Purpura

Discussion

Member takes

Implication

Key Points

Cite This Study

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