What question did this study set out to answer?

Identify the phenotypic profile and specific anatomical distribution of primary nonhematopoietic mesenchymal stem cell subsets within human bone marrow.

March 18, 2011BloodOpen Access

CD146 expression on primary nonhematopoietic bone marrow stem cells is correlated with in situ localization

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Authors

ATAriane TorminLund University LOLi OuResearch Institute of Petroleum Exploration and Development JBJan C. BruneGerman Institute for Cell and Tissue Replacement

Discussion

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Implication

Laboratory analysis reveals CD146 expression stratifies perivascular versus bone-lining human bone marrow stem cells, indicating an oxygen-dependent spatial niche organization.

Key Points

Identify the phenotypic profile and specific anatomical distribution of primary nonhematopoietic mesenchymal stem cell subsets within human bone marrow.
Sorted primary human bone marrow mesenchymal stem cells into distinct subpopulations based on lin, CD271, CD45, and CD146 cell-surface expression.
Assayed colony-forming unit-fibroblast capacity and evaluated bone and hematopoietic stroma formation in vivo.
Examined CD146 expression under normoxic versus hypoxic conditions and mapped in situ spatial relationships with CD34⁺ hematopoietic stem/progenitor cells.
Colony-forming units-fibroblast were exclusively enriched in lin⁻/CD271⁺/CD45⁻ cells regardless of whether CD146 was positive or negative/low, with both populations forming bone and hematopoietic stroma in vivo.
CD146 expression was up-regulated in normoxia and down-regulated in hypoxia, matching in situ tissue distribution where CD146⁺ cells localized to perivascular areas and CD146⁻ cells lined bone surfaces.
CD34⁺ hematopoietic stem and progenitor cells were located in close physical proximity to mesenchymal stem cells in both perivascular and endosteal compartments.

Cite This Study

Tormin et al. (2011) studied this question.

synapsesocial.com/papers/6a98bd19b0871bb756e10774 https://doi.org/10.1182/blood-2010-08-304287

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March 18, 2011BloodOpen Access

CD146 expression on primary nonhematopoietic bone marrow stem cells is correlated with in situ localization

View Full Paper

Ask AI

Bookmark

Authors

ATAriane TorminLund University LOLi OuResearch Institute of Petroleum Exploration and Development JBJan C. BruneGerman Institute for Cell and Tissue Replacement

Discussion

Member takes

Implication

Laboratory analysis reveals CD146 expression stratifies perivascular versus bone-lining human bone marrow stem cells, indicating an oxygen-dependent spatial niche organization.

Key Points

Identify the phenotypic profile and specific anatomical distribution of primary nonhematopoietic mesenchymal stem cell subsets within human bone marrow.
Sorted primary human bone marrow mesenchymal stem cells into distinct subpopulations based on lin, CD271, CD45, and CD146 cell-surface expression.
Assayed colony-forming unit-fibroblast capacity and evaluated bone and hematopoietic stroma formation in vivo.
Examined CD146 expression under normoxic versus hypoxic conditions and mapped in situ spatial relationships with CD34⁺ hematopoietic stem/progenitor cells.
Colony-forming units-fibroblast were exclusively enriched in lin⁻/CD271⁺/CD45⁻ cells regardless of whether CD146 was positive or negative/low, with both populations forming bone and hematopoietic stroma in vivo.
CD146 expression was up-regulated in normoxia and down-regulated in hypoxia, matching in situ tissue distribution where CD146⁺ cells localized to perivascular areas and CD146⁻ cells lined bone surfaces.
CD34⁺ hematopoietic stem and progenitor cells were located in close physical proximity to mesenchymal stem cells in both perivascular and endosteal compartments.

Cite This Study

Tormin et al. (2011) studied this question.

synapsesocial.com/papers/6a98bd19b0871bb756e10774 https://doi.org/10.1182/blood-2010-08-304287

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