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Identify and validate stable reference genes for normalizing qRT-PCR data during flower development in Lagerstroemia indica and Lagerstroemia speciosa.

March 26, 2018PLoS ONEOpen Access

Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa

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Authors

TZTangchun ZhengBeijing Forestry University ZCZhilin ChenLishui University YJYiqian JuQingdao Agricultural University

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Implication

Validation study reveals RPII and EF-1α as optimal reference genes in Lagerstroemia, highlighting reliable targets for gene expression analysis during flower development.

Key Points

Identify and validate stable reference genes for normalizing qRT-PCR data during flower development in Lagerstroemia indica and Lagerstroemia speciosa.
Assessed the expression stability of nine candidate reference genes (GAPDH, TUA, TUB, 18S, RPII, EF-1α, ACT, EIF5A, and CYP) across tissues and flower developmental stages in L. indica and L. speciosa.
Evaluated stability rankings using four statistical algorithms: geNorm, NormFinder, BestKeeper, and RefFinder.
Validated the accuracy of selected reference genes by analyzing the expression profile of the Arabidopsis thaliana AGAMOUS homolog (LsAG1).
RPII and EF-1α showed the highest expression stability across both Lagerstroemia species during floral development.
ACT exhibited high stability specifically in L. indica, while GAPDH was identified as a suitable reference gene for L. speciosa.
TUB demonstrated substantial expression variance across all evaluated samples, making it unsuitable for gene expression normalization.

Cite This Study

Zheng et al. (2018) studied this question.

synapsesocial.com/papers/6a98cc91555fc9bb84748966 https://doi.org/10.1371/journal.pone.0195004

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Implication

Validation study reveals RPII and EF-1α as optimal reference genes in Lagerstroemia, highlighting reliable targets for gene expression analysis during flower development.

Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa

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Implication

Key Points

Cite This Study

Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa

Discussion

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Implication

Key Points

Cite This Study

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