August 1, 1996Journal of Biological ChemistryOpen Access

β-Galactosidase Enzymatic Activity as a Molecular Probe to Detect Specific Antibodies

View Full Paper

Ask AI

Bookmark

Population

Recombinant beta-galactosidase proteins with inserted antigenic region of foot-and-mouth disease virus…

Design

Preclinical

Authors

ABAntoni BenitoUniversitat de Girona JFJordi X. Feliu AVAntonio VillaverdeUniversitat Autònoma de Barcelona

Discussion

Member takes

Implication

May support enzyme-based antibody assays; leaves open clinical translation beyond preclinical models.

Structured PICO

Population

Recombinant beta-galactosidase proteins with inserted antigenic region of foot-and-mouth disease virus serotype C1 (site A)

Intervention

Binding of specific antibodies (monoclonal and polyclonal sera)

Outcome

Beta-galactosidase enzymatic activitysurrogate

Insertion of viral antigenic peptides into beta-galactosidase allows for rapid colorimetric detection of specific antibodies via enzymatic modulation.

Cite This Study

Benito et al. (1996) studied this question.

synapsesocial.com/papers/6a98d45e555fc9bb84748aae https://doi.org/10.1074/jbc.271.35.21251

View Full Paper

Ask AI

Bookmark

β-Galactosidase Enzymatic Activity as a Molecular Probe to Detect Specific Antibodies

Discussion

Member takes

Implication

Structured PICO

Cite This Study

β-Galactosidase Enzymatic Activity as a Molecular Probe to Detect Specific Antibodies

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider