The influence of gas atmosphere packaging (1) 15% CO 2 , 40% 0 2 , 45% N 2 ; (2) 60% CO 2 40% 0 2 (3) 10% CO 2 , 5% 0 2 , 85% N 2 ; (4) film overwrap (control) on the microbial growth and succession on steaks was studied during storage. Treatment 3 was most effective in reducing psychrotrophic growth on steaks. Pseudomonas species dominated the microflora for all packaging treatments during early storage. In all gas atmospheres. Serratia liquefaciens increased with storage time and the 10% CO 2 atmosphere showed the greatest increase. Enterobacter aerogenes appeared late in the storage period in all atmospheres and Yersinia enterocolitica was isolated after 12 days of storage from steaks in atmosphere 1.
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Ahmad et al. (1989) studied this question.