(1→3)-α-d-Glucan from Fruiting Body and Mycelium ofCerrena unicolor(Bull.) Murrill: Structural Characterization and Use as a Novel Inducer of Mutanase

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Water-insoluble, alkali-soluble polysaccharide (marked as ASP) was extracted from the vegetative mycelium and fruiting body ofCerrena unicolorstrain. Monosaccharide examination of ASP demonstrated that the isolated biopolymer was composed mainly of glucose, xylose, and mannose monomers. The methylation investigation of studied polymers indicated that (1→3)-linked α -D-Glcpis the major chain constituent (92.2% for glucans isolated from fruiting body and 90.1% from mycelium).1H NMR, FT-IR, and immunofluorescent labelling determinations confirmed that the polysaccharides isolated from both fruiting body and mycelium of C . unicolorare (1→3)- α -d-glucans. The obtained (1→3)- α -d-glucans showed differences in viscosity and similar characteristics in optical rotations. (1→3)- α -d-Glucans extracted from mycelium and fruiting body ofC. unicolorwere also used as potential and specific inducers of mutanase synthesis byTrichoderma harzianum. The highest mutanase activity (0.38 U/mL) was obtained after induction of enzyme by (1→3)- α -d-glucan isolated from the mycelium ofC. unicolor, and this biopolymer has been suggested as a new alternative to streptococcal mutan for the mutanase induction inT. harzianum. (1→3)- α -d-Glucan-induced mutanase showed high hydrolysis potential in reaction with dextranase-pretreated mutan, where maximal degree of saccharification and solubilization of this bacterial homoglucan (83.1% and 78.4%, resp.) was reached in 3 h at 45°C.