What question did this study set out to answer?

Investigate the three-dimensional structural reorganization of the Golgi apparatus in wild-type and temperature-sensitive sec7 mutant Saccharomyces cerevisiae during secretory blockage and recovery.

December 1, 1993The Anatomical Record

Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three‐dimensional electron microscopy

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Authors

ARA. RambourgCentre National de la Recherche Scientifique YCY. ClermontPacific Northwest Diabetes Research Institute FKFrançois KépèsGoogle (United States)

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Implication

Experimental microscopy study reveals reversible formation of stacked Golgi saccules during secretion block in yeast sec7 mutants, indicating Golgi stacks accumulate when granule release halts.

Key Points

Investigate the three-dimensional structural reorganization of the Golgi apparatus in wild-type and temperature-sensitive sec7 mutant Saccharomyces cerevisiae during secretory blockage and recovery.
Cultured wild-type and sec7 mutant yeast cells at the nonpermissive temperature (37°C) in low-glucose medium, followed by return to the permissive temperature (24°C).
Prepared thick cell sections using glutaraldehyde fixation and reduced osmium postfixation to enhance Golgi content contrast for three-dimensional electron microscopy.
Wild-type cells displayed isolated networks of branching tubules with secretory granule-sized distensions, whereas sec7 mutants at 37°C lost secretory granules entirely.
Blocked sec7 mutants developed stacks of seven to eight flattened, interconnected saccules lacking small vesicles, which reverted to tubular networks upon temperature reduction to 24°C.

Cite This Study

Rambourg et al. (1993) studied this question.

synapsesocial.com/papers/6a98faccea8974fe8c2730bd https://doi.org/10.1002/ar.1092370402

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Implication

Experimental microscopy study reveals reversible formation of stacked Golgi saccules during secretion block in yeast sec7 mutants, indicating Golgi stacks accumulate when granule release halts.

Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three‐dimensional electron microscopy

Discussion

Member takes

Implication

Key Points

Cite This Study

Modulation of the Golgi apparatus in Saccharomyces cerevisiae sec7 mutants as seen by three‐dimensional electron microscopy

Discussion

Member takes

Implication

Key Points

Cite This Study

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