A fiberoptic reflectance probe with multiple source-collector separations to increase the dynamic range of derived tissue optical absorption and scattering coefficients

Measurement of tissue optical absorption and (transport) reduced scattering coefficients (mu(a) and mu(s)', respectively) is fundamental to many applications of light in medicine and biology. We report a handheld fiberoptic probe to determine these coefficients by measuring the diffuse reflectance at multiple source-collector distances, which allows for a larger dynamic range than a single source-collector separation. Diffusion theory and a priori knowledge of the spectral shape of mu(a) and mu(s)' are used in a forward model of the diffuse reflectance. The dynamic range and accuracy of this method were evaluated using Monte Carlo simulations, phantom experiments and tissues in vivo.