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A method of DNA analysis has been developed to verify the authenticity of grouper ( Epinephelus guaza ), wreck fish ( Polyprion americanus ), and Nile perch ( Lates niloticus ) fillets. A short fragment (208 bp) of the mitochondrial 12S rRNA gene was amplified by the polymerase chain reaction and analyzed by single-strand conformation polymorphism to get species-specific patterns of single-stranded DNA (ssDNA). DNA strands were separated by native polyacrylamide gel electrophoresis and visualized by silver staining. Discrimination among the three fish species studied was possible, because each one expressed a specific ssDNA pattern.
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Asensio et al. (2001) studied this question.
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