The Boehringer one-component high-performance cholesterol oxidase reagent has been modified by the inclusion of 2,4,6-tribromo-3-hydroxybenzoic acid (TBHBA) to give a fourfold increase in sensitivity to a molar absorbance of approximately equal to 29,000 with respect to cholesterol. The resulting reagent system is particularly suitable for the determination of plasma HDL-cholesterol for which a reagent of high sensitivity is required. A simple method of bromination avoiding the use of elemental bromine is used to prepare TBHBA. The modified reagent system has been found to have good within- and between-batch precision and has shown itself to be reliable and trouble-free.
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Trinder et al. (1984) studied this question.
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