The effect of human adipose-derived multipotent mesenchymal stromal cells in the fibrin gel on the healing of full-thickness skin excision wounds in mice

Prospects for the widespread use of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine determine the relevance of studying their abilities to affect the reparative process in experimental systems in vivo.Materials and methods. The effect of human adipose-derived MSCs on the healing rate and completeness of damaged skin site reconstitution was examined using full-thickness excision wound model in mice. The reparative activity of MSCs was revealed in planimetric and histological studies. Human blood plasma-derived fibrin gel was used as a scaffold for MSCs delivery.Results and conclusions. Compared to the spontaneous healing process, application of fibrin gel on the excisional skin wounds promotes earlier maturation of granulation tissue and further formation of loose scar tissue with skin derivates. MSCs in the fibrin gel contribute to the improve of wound epithelialization, the decrease of the inflammatory response, faster maturation of the granulation tissue, including marks of angiogenesis, as well as promotes complete recovery of the dermal and epidermal layers of the damaged site of skin.