Why the study?
Do NSAIDs (indomethacin, piroxicam, ibuprofen) inhibit prostaglandin and 12-HETE formation in inflamed human gingival tissues in vitro?
Do NSAIDs (indomethacin, piroxicam, ibuprofen) inhibit prostaglandin and 12-HETE formation in inflamed human gingival tissues in vitro?
Indomethacin, piroxicam, and ibuprofen dose-dependently inhibit prostaglandin and 12-HETE production in inflamed human gingival tissue in vitro.
NSAIDs inhibit gingival PGs and 12-HETE in vitro; hypothesis-generating and leaves open clinical translation to periodontitis.
We investigated the effect of non-steroidal anti-inflammatory drugs on prostaglandins (PGs) and 12-hydroxyeicosatetraenoic acid (12-HETE) formation by inflamed human gingival tissues. Gingival tissue homogenates were incubated with 14C-arachidonic acid in the presence of indomethacin, piroxicam, or ibuprofen, and the organic solvent extracts were chromatographed on silica gel plates with standards for radiometric assay. There was a significant negative trend between the doses (10(-7)-10(-3) M) of each of indomethacin, piroxicam, and ibuprofen, and the amounts of PGF2 alpha, PGE2, PGD2, and 15-keto-PGE2 produced. All three drugs have a significant inhibitory effect on PGs and 12-HETE production at 10(-3) M when compared with the control. The rank order effectiveness of the drugs, at 10(-3) M, on PG inhibition was indomethacin greater than piroxicam greater than ibuprofen, and on 12-HETE inhibition was indomethacin greater than ibuprofen greater than piroxicam.
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ElAttar et al. (1983) studied this question.
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