Why the study?
The study aimed to analyze the improvement mechanism of visceral hypersensitivity and therapeutic targets of Shugan Jiangni Hewei granules for nonerosive reflux disease treatment.
Does Shugan Jiangni Hewei granules (SJHG) alter protein expression in the anterior cingulate cortex of rats with nonerosive reflux disease?
Does Shugan Jiangni Hewei granules (SJHG) alter protein expression in the anterior cingulate cortex of rats with nonerosive reflux disease?
SJHG may treat visceral hypersensitivity in nonerosive reflux disease by modulating immune response and neural signal transmission via Kng1, JAM-A, and the PI3K/Akt pathway.
Objective: The current study aims to analyze the improvement mechanism of visceral hypersensitivity (VH) and targets of Shugan Jiangni Hewei granules (SJHG) for nonerosive reflux disease (NERD) treatment as well as to offer an experimental foundation for its clinical use. Methods: = 36) were acquired in the current study that was further split into three groups: blank, model, and drug (SJHG). Subsequently, differentially expressed proteins and bioinformatics analysis were performed on the collected tissue samples acquired from the anterior cingulate cortex of the model and SJHG rat groups using a tandem mass tag- (TMT-) based proteomics. Eventually, the obtained data from the bioinformatic analysis was further verified through western blotting. Results: From the bioinformatics analysis, only 64 proteins were differentially expressed between the NC and SJHG groups. These molecules were found to be highly expressed in immunological response and neural signal transmission. Finally, we confirmed three therapeutic targets of SJHG, namely, kininogen 1 (Kng1), junctional adhesion molecule A (JAM-A), and the PI3K/Akt signaling pathway. Conclusions: SJHG is effective in treating VH, Kng1 and JAM-A may be therapeutic targets of SJHG, and the therapeutic mechanism of SJHG may be realized by influencing immune response or transmission of neural signals.
No takes yet. Share an insight, caveat, or question.
Wang et al. (2022) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: