An ATF2-derived peptide sensitizes melanomas to apoptosis and inhibits their growth and metastasis

MethodsCells.SW1 and B16F10 mouse melanoma cells were maintained in DMEM supplemented with 10% FBS, L-glutamine, and antibiotics.Constructs.ATF2 peptides were cloned in frame into a hemagglutinpenetratin (HA-penetratin) pcDNA3 vector (6).ATF2 peptide, corresponding to amino acids 50-100 of human ATF2, shares 100% homology with the murine sequence (corresponding amino acids in the mouse ATF2 cDNA are 33-82).Jun2luciferase (Jun2-luc) and TRE-luc constructs were previously described (8, 10).The ATF2 50-100 peptide was