Development of Mucoadhesive Nanofiber as an Alternate Therapeutic Regimen for Subclinical Endometritis in Cattle

Objectives: Subclinical endometritis in cattle is still a problem that has a detrimental effect on dairy production and fertility. Traditional intrauterine or systemic antibiotic treatments frequently result in suboptimal uterine drug levels, encourage resistance, and increase the possibility of drug residues in food items. The current study aimed to treat subclinical endometritis in repeat breeders by creating and testing an ofloxacin (Ofx)-loaded mucoadhesive polyvinyl alcohol/chitosan nanofiber using the electrospinning technique. Materials and Methods: (endometrial cytology and plasma drug concentration) parameters were evaluated. Results: studies showed no cytotoxic effects. In vivo studies on the cattle suggested suitability for localized intrauterine administration. These findings indicate that encapsulation of Ofx in nanofibers stabilises the formulation and offers enhanced treatment efficiency compared to a conventional treatment regimen for subclinical endometritis in repeated breeders. Conclusion: validation, long-term reproductive effects, and resistance management to translate into practical veterinary uses.