In vitro analysis reveals varied dextran structures and elevated viscosity among sugarcane Leuconostoc isolates, indicating significant impacts on juice filtration and crystallization.
Key Points
Characterize exopolysaccharides produced by factory-derived Leuconostoc strains to evaluate their structural features and operational impacts on sugarcane processing viscosity.
Characterized exopolysaccharides isolated from Leuconostoc suionicum (LASM5, LASM6, LASM7) and Leuconostoc lactis (LASM16) using FTIR, NMR spectroscopy, and glycosidic linkage analysis.
Profiled the genomes of all four isolate strains to identify glucansucrase-encoding genes responsible for dextran synthesis.
L. suionicum LASM7 and L. lactis LASM16 exhibited elevated culture viscosity, driven by dextrans enriched with linear α-1,6-linked glucopyranosyl residues.
L. suionicum LASM5 and LASM6 generated dextrans containing 4-linked glucopyranosyl residues on disaccharide branches and a minor 6-linked β-Glc p component.
Genomic profiling revealed multiple branching glucansucrases (Gtf1624, BsrA, DsrE homologs) in LASM5, LASM6, and LASM7, whereas LASM16 encoded a single linear α-1,6-dextransucrase.