Experimental study demonstrates efficient bovine satellite cell isolation using electric mincing and magnetic sorting, highlighting a scalable approach for cultivated meat production.
Background Satellite cells are the primary drivers of skeletal muscle growth and regeneration and are a major cell source in both muscle tissue engineering and cultivated meat. However, current bovine satellite cell isolation can be technically demanding or yield heterogeneous cell populations. Methods This study presents a workflow optimized for simplicity and cost‐efficiency by integrating rapid muscle processing using electric mincing with magnetic‐activated cell sorting targeting Integrin‐α7 to reliably enrich satellite cells. The isolated cells were characterized by Pax7 immunostaining and myogenic differentiation assays. Results ITGA7‐based magnetic sorting yielded an enriched satellite cell population corresponding to 49.39 ± 14.70 × 10³ cells per gram of minced tissue. The isolated cells exhibited high Pax7 expression (98 ± 1.34% Pax7‐positive cells) and strong myogenic differentiation capacity (differentiation index: 94.39 ± 3.58%), confirming their identity and functional potential. Conclusion By minimizing hands‐on effort and avoiding specialized equipment, this workflow lowers technical and financial barriers while enabling the acquisition of highly enriched, early‐passage satellite cell populations. This workflow provides an adaptable foundation for advancing cultivated meat bioprocess development and accelerating research in skeletal muscle biology.
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Baldeweg et al. (2026) studied this question.
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