Key result
The MARPLES platform accurately diagnosed HFMD and identified influenza A subtypes within an hour, demonstrating 100% predictive agreement with qRT-PCR.
Why the study?
Controlling infectious diseases caused by multiple pathogens or subtypes requires multiplex nucleic acid detection platforms capable of rapid, specific, sensitive, and simultaneous gene detection.
Does MARPLES accurately diagnose HFMD and influenza A compared to qRT-PCR?
Does MARPLES accurately diagnose HFMD and influenza A compared to qRT-PCR?
MARPLES is a rapid and accurate multiplex nucleic acid detection platform for infectious disease diagnosis and pathogen identification.
Answers are grounded in this paper and cite their sources.
May support rapid point-of-care HFMD and influenza A diagnosis; leaves open larger validation before routine adoption.
Controlling and mitigating infectious diseases caused by multiple pathogens or pathogens with several subtypes require multiplex nucleic acid detection platforms that can detect several target genes rapidly, specifically, sensitively, and simultaneously. Here, we develop a detection platform, termed Multiplex Assay of RPA and Collateral Effect of Cas12a-based System (MARPLES), based on multiplex nucleic acid amplification and Cas12a ssDNase activation to diagnose these diseases and identify their pathogens. We use the clinical specimens of hand, foot, and mouth disease (HFMD) and influenza A to evaluate the feasibility of MARPLES in diagnosing the disease and identifying the pathogen, respectively, and find that MARPLES can accurately diagnose the HFMD associated with enterovirus 71, coxsackievirus A16 (CVA16), CVA6, or CVA10 and identify the exact types of H1N1 and H3N2 in an hour, showing high sensitivity and specificity and 100% predictive agreement with qRT-PCR. Collectively, our findings demonstrate that MARPLES is a promising multiplex nucleic acid detection platform for disease diagnosis and pathogen identification.
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Lin et al. (2023) studied Hand, foot, and mouth disease (HFMD) and influenza A. MARPLES (Multiplex Assay of RPA and Collateral Effect of Cas12a-based System) vs. qRT-PCR was evaluated on Predictive agreement with qRT-PCR. The MARPLES platform accurately diagnosed HFMD and identified influenza A subtypes within an hour, demonstrating 100% predictive agreement with qRT-PCR.
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