What is the clinical evidence from this study?

Study design: Other. Intervention: Alamandine vs. Vehicle. Primary outcome: Blood serum leptin levels (ng/mL) (p=<0.05).

June 7, 2017PLoS ONEOpen Access

Alamandine reduces leptin expression through the c-Src/p38 MAP kinase pathway in adipose tissue

Q: What are the key findings of this study?

Alamandine significantly reduced blood serum leptin levels and decreased leptin expression in adipose tissue compared to vehicle in male Wistar rats.

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Key result

Alamandine significantly reduced blood serum leptin levels and decreased leptin expression in adipose tissue compared to vehicle in male Wistar rats.

Population

Male Wistar rats, isolated peri-renal visceral adipose tissue, isolated visceral adipocytes, and mouse…

Comparison

Alamandine and Angiotensin 1-7 vs Vehicle (saline) or PBS control

Design

Preclinical

Follow-up

24 hours

Authors

TUTsuyoshi UchiyamaSeirei Hamamatsu General Hospital FOFumikazu OkajimaAomori University CMChihiro MogiGunma University

Discussion

Member takes

Implication

Alamandine lowers leptin in animal adipocytes; hypothesis-generating for MrgD pathways but leaves open human translation or clinical relevance.

Structured PICO

Population

Male Wistar rats aged 10-13 weeks were used to study the effects of alamandine on isolated peri-renal visceral adipose tissue and adipocytes.

Intervention

Alamandine (1 nM in vitro, 5.76 μg/kg intraperitoneally in vivo) and Angiotensin 1-7 (1 nM to 1000 nM in vitro, 5.76 μg/kg in vivo)

Comparator

Vehicle (saline) or PBS control

Outcome

Leptin expression (mRNA) and secretion (protein levels in blood and tissue)surrogate

Main Result

Absolute Event Rate: 2.01% vs 2.56%

p-value: p=<0.05

Alamandine reduces leptin expression and secretion in adipocytes via the MrgD receptor and c-Src/p38 MAP kinase pathway, opposing the effects of low-dose angiotensin 1-7.

Limitations

Alamandine levels have not been determined in humans.
The metabolic cascade of alamandine is not well understood.
MrgD signal transduction remains unclear except for calcium release and phosphokinase A activation.

Cite This Study

Uchiyama et al. (2017) studied this question. Alamandine vs. Vehicle was evaluated on Blood serum leptin levels (ng/mL) (p=<0.05). Alamandine significantly reduced blood serum leptin levels and decreased leptin expression in adipose tissue compared to vehicle in male Wistar rats.

synapsesocial.com/papers/6a993ef7bd35225844190825 https://doi.org/10.1371/journal.pone.0178769

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Implication

Alamandine lowers leptin in animal adipocytes; hypothesis-generating for MrgD pathways but leaves open human translation or clinical relevance.

Alamandine reduces leptin expression through the c-Src/p38 MAP kinase pathway in adipose tissue

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Alamandine reduces leptin expression through the c-Src/p38 MAP kinase pathway in adipose tissue

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Also Consider

Also Consider