What question did this study set out to answer?

To evaluate the in vitro toxic effects of varying cisplatin concentrations on the viability of mouse acute lymphoblastic leukaemia cells and neonatal mouse spermatogonial stem cells.

October 1, 2015AndrologiaOpen Access

In vitrotoxicity assay of cisplatin on mouse acute lymphoblastic leukaemia and spermatogonial stem cells

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Authors

RSRonak ShabaniIran University of Medical Sciences KAKhadijeh AshtariIran University of Medical Sciences BBBabak BehnamAmerican University

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Implication

In vitro study demonstrates dose-dependent toxicity of cisplatin in mouse leukaemia and spermatogonial stem cells, highlighting potential preservation of stem cell colony formation.

Key Points

To evaluate the in vitro toxic effects of varying cisplatin concentrations on the viability of mouse acute lymphoblastic leukaemia cells and neonatal mouse spermatogonial stem cells.
Isolated neonatal mouse spermatogonial stem cells (SSCs) and EL-4 leukaemia cells were assigned to six groups: untreated control, DMSO sham, and cisplatin at 0.5, 5, 10, and 15 μg/ml.
Cell viability was assessed using MTT assay, cell phenotypes were verified with specific markers, and apoptotic pathways were examined via TUNEL assay and expression of BAX and caspase-3.
Viability of both SSCs and EL-4 cells was significantly reduced at 15 μg/ml compared with control (P ≤ 0.05).
Statistically significant differences occurred between IC50 values at doses of 10 and 15 μg/ml across exposure times (P ≤ 0.05).
Effective cisplatin concentrations increased TUNEL-positive apoptotic cells and upregulated BAX and caspase-3 in EL-4 cells, while surviving SSCs retained the capacity to form colonies in culture.

Cite This Study

Shabani et al. (2015) studied this question.

synapsesocial.com/papers/6a993f741c4dac29babd5dbf https://doi.org/10.1111/and.12490

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Implication

In vitro study demonstrates dose-dependent toxicity of cisplatin in mouse leukaemia and spermatogonial stem cells, highlighting potential preservation of stem cell colony formation.

In vitrotoxicity assay of cisplatin on mouse acute lymphoblastic leukaemia and spermatogonial stem cells

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Cite This Study

In vitrotoxicity assay of cisplatin on mouse acute lymphoblastic leukaemia and spermatogonial stem cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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