What question did this study set out to answer?

To identify, sequence, and functionally characterize the gene encoding a thermostable heme O synthase from the thermophilic bacterium Bacillus PS3.

March 31, 2002Medical Entomology and ZoologyOpen Access

Nucleotide Sequence and Characterization of the Gene Coding for a Thermostable Heme O Synthase from the Thermophilic Bacillus PS3

View Full Paper

Ask AI

Bookmark

Authors

MIMorio IshizukaApple (Israel)TNTsuyoshi NakajimaShibaura Institute of Technology MCMakoto Chikira

Discussion

Member takes

Implication

Experimental study reveals the sequence and functional expression of thermostable heme O synthase in Bacillus PS3, highlighting its role in cytochrome oxidase assembly.

Key Points

To identify, sequence, and functionally characterize the gene encoding a thermostable heme O synthase from the thermophilic bacterium Bacillus PS3.
Isolated the caaF-caaE-caaABCD gene cluster from a genomic DNA library of Bacillus PS3.
Sequenced and mapped caaE and its surrounding regulatory regions, followed by sequence alignment with bacterial homologues.
Expressed the gene cluster and caaE heterologously in Escherichia coli to assay membrane-associated enzymatic activity and heme A synthesis.
Determined that caaE encodes a 309-amino-acid protein homologous to E. coli CyoE, situated upstream of caaA alongside a promoter-like stem-loop structure.
Demonstrated that recombinant expression of caaE in E. coli yields functional, thermostable protoheme IX farnesyltransferase activity in the cytoplasmic membrane.
Identified conserved amino acid residues potentially responsible for prenyl binding, transfer, heme B binding, and thermal structural stability.

Cite This Study

Ishizuka et al. (2002) studied this question.

synapsesocial.com/papers/6a9941cf55a4ce6507f5aad4 https://doi.org/10.24789/00001252

View Full Paper

Ask AI

Bookmark

Implication

Experimental study reveals the sequence and functional expression of thermostable heme O synthase in Bacillus PS3, highlighting its role in cytochrome oxidase assembly.

Nucleotide Sequence and Characterization of the Gene Coding for a Thermostable Heme O Synthase from the Thermophilic Bacillus PS3

Discussion

Member takes

Implication

Key Points

Cite This Study

Nucleotide Sequence and Characterization of the Gene Coding for a Thermostable Heme O Synthase from the Thermophilic Bacillus PS3

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider