The fatty acid and glycerol content of glycerides were estimated by a simplified hydrogenolysis–acetylation procedure and GLC. When fatty acids interfered in the analysis, glycerol alone was estimated by saponification–acetylation and GLC. Preliminary acetolysis was necessary for the estimation of glycerol in ethanolamine phosphatides by these methods.
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Holla et al. (1964) studied this question.
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