The immobilisation of enzymes on titanium‐activated inorganic supports

Methods are described for the immobilisation of enzymes on inorganic supports activated by the surface deposition of an imperfectly crystallised film of TiO2. The stability of the preparations is shown to be strongly dependent on the integrity of the support‐TiO2 link which is in turn dependent on the pretreatment of the solid support. The enzyme may be linked to the activated support using 5‐aminosalicylic acid (with or without diazotisation), tannic acid or stannous chloride, or by direct contact. These preparations are shown to have activities and operational stabilities superior to those of a preparation made using the silane‐glutaraldehyde method on the same glass support.