Answers are grounded in this paper and cite their sources.
More rapid than with conventional methods is the analysis of ribozyme kinetics upon use of FRET substrates. In these substrates a fluorophore and a fluorescence-quenching molecule lie in close spatial proximity. The intramolecular fluorescence quenching is neutralized upon cleavage, and the fluorescence intensity is a measure of the ribozyme activity. By automation and computer assistance the activity of ribozymes can be monitored under high-throughput conditions.
No takes yet. Share an insight, caveat, or question.
Jenne et al. (1999) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: