Key result
Cloning and sequencing of an altered Rous sarcoma virus provirus in mammalian tumor cell lines demonstrated reverse transcription and normal retroviral integration of src mRNA.
The study demonstrates reverse transcription and normal retroviral integration of src mRNA in mammalian cells, generating an LTR, v-src, LTR provirus.
Different types of altered proviruses of Rous sarcoma virus (RSV) have been detected in mammalian tumor cell lines. We cloned and sequenced one of these altered proviruses with the structure LTR, v-src, LTR. The presence of an intact viral splice junction, as well as duplications of the chromosomal sequence GCGGGG flanking the two 2-base-pair-deleted LTRs, demonstrated reverse transcription and normal retroviral integration of src mRNA in mammalian cells. In addition, a 1-nucleotide deletion 2 bases upstream from the AAUAAA polyadenylation signal is suspected to be responsible for the absence of a poly(A) track in the src mRNA present in virions of rescued viruses.
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Bodor et al. (1989) studied mammalian tumor cell lines. Cloning and sequencing of altered RSV provirus was evaluated on Structure of altered provirus and evidence of reverse transcription and integration. Cloning and sequencing of an altered Rous sarcoma virus provirus in mammalian tumor cell lines demonstrated reverse transcription and normal retroviral integration of src mRNA.
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