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April 1, 1989Journal of VirologyOpen Access

Multiple regions in the Rous sarcoma virus src gene intron act in cis to affect the accumulation of unspliced RNA

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Population

Chicken embryo fibroblasts

Comparison

Transfection with mutant Rous sarcoma virus DNA… vs Transfection with wild-type Rous sarcoma virus…

Design

Preclinical

Authors

CSC. Martin StoltzfusUniversity of IowaSFSteven J. FogartyMerck & Co., Inc., Rahway, NJ, USA (United States)

Discussion

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Implication

Hypothesis-generating for retroviral splicing control; leaves open conservation or therapeutic relevance in human systems.

Structured PICO

P
Population
Chicken embryo fibroblasts
I
Intervention
Transfection with mutant Rous sarcoma virus DNA clones (deleted in src intron or gag gene region)
C
Comparator
Transfection with wild-type Rous sarcoma virus DNA clone
O
Outcome
Ratio of unspliced to spliced viral RNAsurrogate

Two cis-acting regions in the Rous sarcoma virus src gene intron are important in the negative control of RNA splicing.

Cite This Study

Stoltzfus et al. (1989) studied this question.

synapsesocial.com/papers/6a8581e9b451cea71dae656chttps://doi.org/10.1128/jvi.63.4.1669-1676.1989
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter1984 · 6,459 citations
  2. 2Noncoding region between the env and src genes of Rous sarcoma virus influences splicing efficiency at the src gene 3' splice site1987 · 32 citations
  3. 3Gene Expression from Both Intronless and Intron-Containing Rous Sarcoma Virus Clones Is Specifically Inhibited by Anti-Sense RNA1985 · 40 citations