Gas chromatographic analysis of fatty acids from dialyzed lipoproteins

Dialysis of three major lipoprotein fractions from human serum, Sf20-105, Sf0-20, and -S1.200-16, against low concentrations of cupric ion is associated with accelerated chemical alteration of polyunsaturated fatty acids and cholesterol. Less extensive changes in these acids were observed during dialysis against ion-free water. Reaction products of these chemical alterations were irreversibly retained on succinic acid-diethylene glycol polyester gas chromatographic columns. Present evidence suggests that these reaction products are relatively polar compounds, probably in the chemical class of lipoperoxides.