An improved 2‐thiobarbituric acid (TBA) method suitable for routine testing of autoxidative changes of fish oil and other polyunsaturated lipids has been developed. Air oxidation of the lipid during the TBA reaction was found to produce misleading results. The air oxidation may be controlled by addition of antioxidants to the reaction system. Other factors causing inconsistent TBA results and methods of prevention are also described. A comparison of peroxide and TBA values in autoxidized menhaden oil is presented.
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Yu et al. (1967) studied this question.
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