Two examples of nanostructured gold surfaces as biosensors. Surface-enhanced chemiluminescence and double detection by surface plasmon resonance and luminescence

In this paper, we present a review of our activities in the field of gold biosensors. Nanostructured gold surfaces can be used in biology for their plasmonic and/or catalytic properties. In a first part, we show how the gold plasmonic properties allow the detection of the biotin-streptavidin binding by two types of techniques: extinction spectroscopy and also luminescence spectroscopy when the probe (here the streptavidin) is labelled by polysiloxane particles encapsulating fluorophores. In a second part, we demonstrate that the catalytical properties of gold corrugated surfaces can significantly enhance the chemiluminescence of luminol brought at vicinity. We found that the surface-enhancement induced by gold is two times of magnitude greater than that induced by silver.