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We report the development of a laser-scanning surface plasmon resonance microscope equipped with multiple photodetectors. The system simultaneously obtains images that have different orientations to the specimen under examination, and the system can achieve a spatial resolution of 1.6 microm, which is shorter than the propagation length of the scanning surface plasmons, while high sensitivity to thickness and refractive-index distribution is retained. In addition, because the system uses a laser-scanning microscope, it can obtain high-contrast images. The measurable ranges of refractive-index distributions and thicknesses of specimens that can be measured are also discussed.
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Tanaka et al. (2003) studied this question.
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