A method is described which allows the identification of individual carbohydrates of multi-component artificial mixtures using the computer-aided analysis of their 13C NMR spectra, without previous separation. Quantitation of mono-, di- and trisaccharides was carried out after improvement of the experimental procedure and using an internal standard. The procedure was then applied to authentic honeys of different floral types, harvested in Corsica (France). Several oligosaccharides (identification of some of these is not easy by chromatographic techniques) were observed at levels ranging from 0.4 to 3.3%. In addition, the fructose/glucose ratio, which has an influence on crystallization, was easily obtainable.
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Mazzoni et al. (1997) studied this question.