What question did this study set out to answer?

To determine the native quaternary structure of the integral membrane protein stomatin and identify the domain responsible for its self-association.

July 1, 1998Journal of Biological ChemistryOpen Access

Oligomeric Nature of the Integral Membrane Protein Stomatin

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Authors

LSLuc SnyersMedical University of Vienna EUEllen UmlaufMedical University of Vienna RPRainer ProhaskaMax Perutz Labs

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Implication

Biochemical analysis reveals that stomatin forms large homo-oligomers in human cells, indicating that its C-terminus drives assembly critical for membrane organization.

Key Points

To determine the native quaternary structure of the integral membrane protein stomatin and identify the domain responsible for its self-association.
Analyzed Triton X-100 soluble extracts from human UAC cells using density gradient centrifugation and chemical cross-linking.
Performed co-immunoprecipitation of endogenous stomatin with recombinantly expressed myc-tagged stomatin using an anti-myc antibody.
Evaluated oligomer incorporation using two recombinant C-terminally truncated stomatin variants.
Stomatin organizes into soluble homo-oligomeric complexes containing between 9 and 12 monomer subunits.
Endogenous stomatin successfully co-immunoprecipitates with recombinant myc-tagged stomatin, confirming self-association in cellular extracts.
Deletion of the stomatin C terminus completely abolishes incorporation into native complexes, establishing its necessity for homo-oligomerization.

Cite This Study

Snyers et al. (1998) studied this question.

synapsesocial.com/papers/6a999f3af8ae396fbf28f89f https://doi.org/10.1074/jbc.273.27.17221

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Implication

Biochemical analysis reveals that stomatin forms large homo-oligomers in human cells, indicating that its C-terminus drives assembly critical for membrane organization.

Oligomeric Nature of the Integral Membrane Protein Stomatin

Discussion

Member takes

Implication

Key Points

Cite This Study

Oligomeric Nature of the Integral Membrane Protein Stomatin

Discussion

Member takes

Implication

Key Points

Cite This Study

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