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March 3, 1986FEBS Letters

Type VI collagen is a major component of the human cornea

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Authors

DZDieter R. ZimmermannUniversity Hospital of ZurichBTBeat TrüebUniversity of BernKWKaspar H. WinterhalterETH Zurich

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Implication

Biochemical analysis reveals substantial amounts of type VI collagen in human cornea, indicating it is a major structural component of the corneal extracellular matrix.

Key Points

  • To determine whether type VI collagen is present in the human cornea and to assess its abundance and tissue distribution.
  • Characterized corneal collagen via comparative peptide mapping against type VI collagen purified from human placenta.
  • Identified and quantified protein levels using antibodies specific for human type VI collagen via immunoblotting and polyacrylamide gel densitometry.
  • Examined spatial localization across corneal tissue sections using indirect immunofluorescence.
  • Immunoblotting and comparative peptide mapping confirmed the structural presence of type VI collagen in human corneal tissue.
  • Polyacrylamide gel scanning revealed that type VI collagen constitutes up to one quarter (25%) of the total dry weight of the cornea.
  • Indirect immunofluorescence demonstrated that type VI collagen is broadly distributed throughout the corneal stroma.

Cite This Study

Zimmermann et al. (1986) studied this question.

synapsesocial.com/papers/6a99a6980e148c09fcabb61ehttps://doi.org/10.1016/0014-5793(86)80297-6
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Characterization of the precursor form of type VI collagen.1984 · 61 citations
  2. 2Isolation of a unique collagenous fraction from limited pepsin digests of human placental tissue. Characterization of one of the constituent polypeptide chains.1980 · 112 citations
  3. 3Immunochemistry, genuine size and tissue localization of collagen VI1984 · 306 citations
  4. 4Type VI collagen. Studies on its localization, structure, and biosynthetic form with monoclonal antibodies.1984 · 208 citations
  5. 5Pepsin-generated type VI collagen is a degradation product of GP140.1984 · 57 citations