Key result
Simultaneous targeting of influenza virus PB2 and NP mRNAs with two external guide sequences inhibited viral protein and particle production by 90% to 100% in cultured mouse cells.
Why the study?
Do external guide sequences targeted to the mRNAs of the PB2 and NP genes inhibit influenza virus production in cultured mouse cells?
Do external guide sequences targeted to the mRNAs of the PB2 and NP genes inhibit influenza virus production in cultured mouse cells?
External guide sequences targeting influenza virus mRNAs can effectively inhibit viral production in cultured cells, with dual targeting being more effective than single targeting.
Dual EGS targeting merits in vivo testing for influenza; leaves open clinical translation.
The polymerase (PB2) and nucleocapsid (NP) genes encoded by the genome of influenza virus are essential for replication of the virus. When synthetic genes that express RNAs for external guide sequences targeted to the mRNAs of the PB2 and NP genes are stably incorporated into mouse cells in tissue culture, infection of these cells with influenza virus is nonproductive. Endogenous RNase P cleaves the targeted influenza virus mRNAs when they are in a complex with the external guide sequences. Targeting two different mRNAs simultaneously inhibits viral particle production more efficiently than does targeting only one mRNA.
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Plehn-Dujowich et al. (1998) studied Influenza virus infection (in vitro). External guide sequences (EGSs) targeting PB2 and NP mRNAs vs. No EGS or mutant EGS was evaluated on Inhibition of influenza virus protein and particle production. Simultaneous targeting of influenza virus PB2 and NP mRNAs with two external guide sequences inhibited viral protein and particle production by 90% to 100% in cultured mouse cells.
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