Key result
Southern blot analysis of MAV-2-infected chicken embryonic fibroblast clones revealed unique sets of junctive bands, indicating that MAV-2 proviruses integrate at multiple sites in cellular DNA.
MAV-2 proviruses integrate at multiple sites in the cellular DNA of chicken embryonic fibroblasts.
Avian cell data should not alter clinical vector safety; leaves open whether multiple integration sites occur in mammalian systems.
The cellular sites of integration of the avian myeloblastosis-associated virus type 2 (MAV-2) DNA have been examined by Southern blot analysis of cellular DNA from infected cloned and uncloned chicken embryonic fibroblasts. Provirus-cell juncture fragments were not detected in restriction enzyme digests of DNA from MAV-2-infected uncloned cells. However, each MAV-2-infected cell clone examined produced a unique set of junctive bands. Thse findings indicate that multiple sites of integration exists for MAV-2 proviruses in cellular DNA.
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Bergmann et al. (1980) studied Avian myeloblastosis-associated virus type 2 (MAV-2) infection. MAV-2 infection was evaluated on Cellular sites of integration of MAV-2 DNA. Southern blot analysis of MAV-2-infected chicken embryonic fibroblast clones revealed unique sets of junctive bands, indicating that MAV-2 proviruses integrate at multiple sites in cellular DNA.
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