Key result
HBoV1 DNA was detected in 12.1% of children with acute bronchiolitis compared to 6% of healthy controls, but viral mRNA indicating active replication was only found in 24.3% of the DNA-positive patients.
Why the study?
The study was conducted to assess the proportion and clinical consequences of human bocavirus 1 replication in children hospitalized for acute bronchiolitis with human bocavirus 1 DNA in the nasopharynx.
Does the detection of HBoV1 mRNA distinguish active viral replication from inactive infection in children hospitalized for acute bronchiolitis?
Observational (n=457)
No
Does the detection of HBoV1 mRNA distinguish active viral replication from inactive infection in children hospitalized for acute bronchiolitis?
Absolute Event Rate: 12.1% vs 6%
p-value: p=0.064
Detection of HBoV1 mRNA, rather than just DNA, along with high viral load and viremia, helps distinguish active HBoV1 infection from asymptomatic shedding in children with acute bronchiolitis.
HBoV1 mRNA detection may mark active replication in DNA-positive bronchiolitis; leaves open its diagnostic or prognostic value in routine care.
BACKGROUND: The objective of this prospective study was to assess the proportion and clinical consequences of human bocavirus 1 (HBoV1) replication in children hospitalized for acute bronchiolitis (AB) with HBoV1 DNA in the nasopharynx (NP). METHODS: For this purpose, we detected HBoV1 DNA and mRNA (evidence of viral replication and viable virus) in NP in cases and healthy control children. This research allowed us to distinguish active HBoV1 infections from inactive ones. RESULTS: HBoV1 DNA was detected in the NP of 37 of 307 patients with AB (12.1%) and 9 of 150 children in a healthy control group (6%) with a high codetection rate with other respiratory viruses in AB patients, 28 of 37 (75.7%). Only 9 of 37 HBoV1 DNA-positive NP swabs (24.3%) with high DNA load were also HBoV1 mRNA positive, moreover, HBoV1 DNA was also detected in the plasma of these patients. CONCLUSIONS: Based on the results of our study, we can conclude that children with AB acute HBoV1 infection has a high HBoV1 DNA load in NP samples together with detected HBoV1 mRNA and detected HBoV1 DNA in plasma.
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Uršič et al. (2024) conducted an observational in Acute bronchiolitis (n=457). Human bocavirus 1 (HBoV1) infection vs. Healthy controls was evaluated on Detection of HBoV1 DNA in nasopharyngeal swabs (p=0.064). HBoV1 DNA was detected in 12.1% of children with acute bronchiolitis compared to 6% of healthy controls, but viral mRNA indicating active replication was only found in 24.3% of the DNA-positive patients.
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