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Cotyledonary development of asexual embryos of cacao ( Theobroma cacao L.) in liquid medium on a rotary drum apparatus was similar to that of zygotic embryos in vivo . Immature zygotic and asexual embryos synthesized anthocyanins, characteristic of the developing zygotic embryo in vivo , when cultured in vitro in liquid or on semisolid media containing high concentrations of sucrose.
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Pence et al. (1981) studied this question.
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