Key result
4D Doppler optical coherence tomography successfully mapped endocardial shear stress in vivo, revealing significantly higher peak shear stress on the inner curvature (7.7 Pa) compared to the outer curvature (2.0 Pa) of the embryonic outflow tract.
Absolute Event Rate: 7.7% vs 2%
p-value: p=0.003
4D Doppler OCT enables accurate, dynamic, and volumetric mapping of endocardial shear stress in the developing embryonic heart in vivo, providing a novel tool to study biomechanical forces in cardiogenesis.
Advances preclinical study of hemodynamic forces in cardiogenesis; leaves open relevance to human congenital defects.
Accurate imaging and measurement of hemodynamic forces is vital for investigating how physical forces acting on the embryonic heart are transduced and influence developmental pathways. Of particular importance is blood flow-induced shear stress, which influences gene expression by endothelial cells and potentially leads to congenital heart defects through abnormal heart looping, septation, and valvulogenesis. However no imaging tool has been available to measure shear stress on the endocardium volumetrically and dynamically. Using 4D structural and Doppler OCT imaging, we are able to accurately measure the blood flow in the heart tube in vivo and to map endocardial shear stress throughout the heart cycle under physiological conditions for the first time. These measurements of the shear stress patterns will enable precise titration of experimental perturbations and accurate correlation of shear with the expression of molecules critical to heart development.
No takes yet. Share an insight, caveat, or question.
Peterson et al. (2012) studied Embryonic heart development (n=3). 4D Doppler optical coherence tomography was evaluated on Peak shear stress on the inner vs outer curvature of the outflow tract (p=0.003). 4D Doppler optical coherence tomography successfully mapped endocardial shear stress in vivo, revealing significantly higher peak shear stress on the inner curvature (7.7 Pa) compared to the outer curvature (2.0 Pa) of the embryonic outflow tract.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: