Key result
The VP7 gene of the atypical human rotavirus M3014 exhibited significant identity to serotype G4 but lacked reactivity with G4 or G9 monoclonal antibodies, suggesting genetic and antigenic drift.
The M3014 VP7 gene likely derived from sequential mutation of a G4-like progenitor gene, resulting in novel antigenic properties.
Antigenic drift in rotavirus VP7 may evade serologic detection; hypothesis-generating for genomic surveillance in human and animal populations.
The nucleotide sequence of the gene encoding the outer capsid glycoprotein, VP7, isolated from a reassortant human rotavirus, M3014, was determined. The deduced amino acid sequence exhibited significant identity to the VP7 from a standard strain belonging to serotype G4, although the antigenic regions of the M3014 VP7 resembled sequences from both serotype G4 and G9 viruses. However, reactivity with G4 or G9 serotype-specific monoclonal antibodies was not observed. We suggest that the M3014 VP7 was derived from sequential mutation of a G4-like progenitor gene resulting in a protein with novel antigenic properties.
No takes yet. Share an insight, caveat, or question.
Palombo et al. (1997) studied Human rotavirus. VP7 gene sequencing of M3014 rotavirus vs. Standard strain serotype G4 and G9 viruses was evaluated on Nucleotide and deduced amino acid sequence identity and antigenic reactivity. The VP7 gene of the atypical human rotavirus M3014 exhibited significant identity to serotype G4 but lacked reactivity with G4 or G9 monoclonal antibodies, suggesting genetic and antigenic drift.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: