Key result
A sandwich ELISA using monoclonal antibody 4D7 and polyclonal rabbit IgG detected feline calicivirus antigen in 88.9% (32 of 36) of swab samples from which the virus was isolated.
A newly developed sandwich ELISA using monoclonal antibody 4D7 can broadly detect feline calicivirus antigen in clinical swab samples, though it remains slightly less sensitive than viral isolation.
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Offers a potential alternative to viral isolation for feline calicivirus detection; leaves open its utility pending further validation.
A monoclonal antibody (MAb) reactive with 36 field isolates and 2 laboratory strains of feline calicivirus (FCV) was produced by immunizing mice with the mixture of FCVs. The MAb (4D7) reacted with FCVs in an enzyme-linked immunosorbent assay (ELISA), but had no neutralizing activity against the F4 strain of FCV. MAb 8G1, previously produced against the FCV F4 strain, also reacted in ELISA with all FCVs used in the present study. However, the epitopes recognized by 4D7 and 8G1 were different. Using these two MAbs and a polyclonal rabbit antibody, we attempted to develop a sandwich ELISA for detection of FCV antigen. The combination of 4D7 and the polyclonal rabbit IgG was most sensitive. Using this system, all the field isolates of FCV cultured in vitro were detected. However, among the 36 swab samples, from which FCV was isolated, 4 were negative.
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Tajima et al. (1998) studied Feline calicivirus infection (n=36). Sandwich ELISA using MAb 4D7 and polyclonal rabbit IgG vs. Virus isolation was evaluated on Detection of FCV antigen in swab samples. A sandwich ELISA using monoclonal antibody 4D7 and polyclonal rabbit IgG detected feline calicivirus antigen in 88.9% (32 of 36) of swab samples from which the virus was isolated.
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