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January 1, 1999Journal of VirologyOpen Access

Feline Calicivirus Capsid Protein Expression and Capsid Assembly in Cultured Feline Cells

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Key result

Expression of feline calicivirus capsid protein using a T7 promoter and MVA/T7pol coinfection resulted in high protein levels, processing of the precursor, and assembly into virus-like particles.

Population

Cultured feline cells (Crandell feline kidney cells [CRFK]), murine L929 cells, and Vero cells

Design

Preclinical

Authors

KGKlaus GeißlerSigmund Freud Privatuniversität WienKSKarla SchneiderEppendorf (Germany)AFAndrea Fleuchaus

Discussion

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Implication

Supports FCV VLP production in mammalian cells; leaves open veterinary vaccine applications pending in vivo validation.

Structured PICO

P
Population
Cultured feline cells (Crandell feline kidney cells [CRFK]), murine L929 cells, and Vero cells
I
Intervention
Plasmids containing cytomegalovirus, simian virus 40, or T7 promoters expressing FCV capsid protein, with or without coinfection with vaccinia virus expressing T7 RNA polymerase (MVA/T7pol)
O
Outcome
FCV capsid protein expression, processing, and assembly into virus-like particlessurrogate

Feline calicivirus precursor capsid protein can be efficiently expressed, processed to mature size, and assembled into empty virus-like particles in cultured mammalian cells using a T7 promoter and MVA/T7pol system.

Limitations

  • The specific proteases involved in the processing of the precursor protein have not yet been identified.
  • Whether the ability to cleave the capsid protein precursor is a host range determinant in vitro cannot be definitively answered.

Cite This Study

Geißler et al. (1999) studied Feline calicivirus (in vitro). Plasmid expression vectors (CMV, SV40, T7 promoters) and MVA/T7pol was evaluated on Capsid protein expression, processing, and assembly. Expression of feline calicivirus capsid protein using a T7 promoter and MVA/T7pol coinfection resulted in high protein levels, processing of the precursor, and assembly into virus-like particles.

synapsesocial.com/papers/6a99d2e57dc1235325abfe42https://doi.org/10.1128/jvi.73.1.834-838.1999
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Nucleotide sequence and expression of the capsid protein gene of feline calicivirus1991 · 120 citations
  2. 2Analysis of feline calicivirus capsid protein genes: identification of variable antigenic determinant regions of the protein1993 · 101 citations
  3. 3Expression and self-assembly of empty virus-like particles of hepatitis E virus1997 · 324 citations
  4. 4Development of Virus-Like Particle Technology from Small Highly Symmetric to Large Complex Virus-Like Particle Structures2013 · 5,966 citations
  5. 5Cleavage of the Feline Calicivirus Capsid Precursor Is Mediated by a Virus-Encoded Proteinase1998 · 127 citations